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## Blind Source Separation in Magnetic Resonance Images

I have been involved in a major project on contrast enhancement of Magnetic Resonance Images by using Independent Component Analysis (ICA) and Support Vector Machines (SVM) for the past couple of  months. It is an extremely exciting project and also something new for me, as I have worked on bio-medical images just once before. In the past, I have used ICA and SVM in face recognition/authentication, however this application is quite novel.

This post intends to introduce the problem, discuss a motivating example, some methods, expected work and some problems.

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A Simple Introduction and Motivating Example:

The simplest motivating example for this problem is the famous cocktail party problem:

You are at a cocktail party, and there are about 12 people present with each talking simultaneously. Add to that a music source. So that makes it 13.

Suppose you want to follow what each person was saying later and for doing so you place a number of tape recorders at different locations in the room (let’s not worry about the number of recorders right now). When you hear them later, the sounds would hardly be understandable as they would be mixed up.

Now you define an engineering problem : that using these recordings (which are basically mixtures), separate out the different sources with as little distortion as possible. In a real time cocktail party, the brain shows[1][2][3] a remarkable ability to follow one conversation. However such a problem has proved to be quite difficult in signal processing. Let’s just illustrate the cocktail party problem in a cartoon below :

The Cocktail Party Problem

Please listen to a demo of the cocktail party problem at the HUT ICA project page.

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The Logic Behind Constructing MR Images in Simple Terms:

Now, keeping the previous brief discussion in mind. Let’s introduce in simple words how MRI works. This is just a simplification to make the idea clearer, and not really how MRI works.  Discussing MRI in detail would divert the focus of the post. To look at how MRI works follow these highly recommended tutorials[4][5][6]:

Suppose your body is placed in a Magnetic Field (let’s not worry about specifics yet). Consider two contiguous tissues in your body – X and Y. When subject to a magnetic field, the particles (protons) in the tissues would get aligned according to the field. The amount of magnetization would depend on the tissue type. Now suppose we want to measure how much a tissue gets magnetized. One way to think about it is like this : First apply the magnetic field, after the application the particles would get excited. Once the field is removed, these particles would tend to relax to their ground state. By being able to measure the time it takes for the particles to return, we would get some measure of the magnetization of the tissue(s). This is because, the greater the time for relaxation, greater the magnetization.

An image is basically a measure of the energy distribution. Now suppose we have the measurements for tissues X and Y, and since they were of a different nature (composition, density of protons etc), their response to the field would have been different. Thus we would get some contrast between them and thus would get an image.

In very simplistic terms, this is how MRI scans are obtained. Though as mentioned above, please follow [4][5][6] for detailed tutorials on MRI.

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MRI scans of the Brain and the Cocktail Party Problem :

Now consider the above discussion in context of taking a MRI scan of the brain. The brain has a number of constituents. Some being : Gray Matter, White Matter, Cerebrospinal Fluid (CSF) Fat, Muscle/Skin, Glial Matter etc. Now since each is unique, they would exhibit unique characteristics under a magnetic field. However, while taking a scan, we get one MRI image of the entire brain.

These scans can be considered as an equivalent to the mixtures of the cocktail party example. If we apply blind source separation to these, we should be able to separate out the various constituents such as gray matter, white matter, CSF etc. These images of the independent sources can be used for better diagnosis. This would be something like this :

If suppose the Simulated MR scans (from the McGill Simulated brain Database) were as follows:

Simulated MR Scans

The “ground truth” images for these scans would be as follows :

Ground Truth Images of Different Brain Tissue Substances

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Restatement of the Broad Research Problem and Use of ICA and SVM:

Magnetic Resonance Imaging is superior to Computerised Tomography for brain imaging at least, for the reason that it can give much better soft tissue contrast (because even small changes in the proton density and composition in the tissue are well represented).

Like for most techniques, improvements to scans obtained by MRI are much desired to improve diagnosis. Blind source separation has been used to separate physiologically different components from EEG[7]/MEG[8] data (similar to the cocktail party problem), financial data[9] and even in fMRI[10][11]. But it has not received much attention for MRI. Nakai et al[12] used Independent Component Analysis for the purpose of separating physiologically independent components from MRI scans. They took MR images of 10 normal subjects, 3 subjects with brain tumour and 1 subject with multiple sclerosis and performed ICA on the data. They reported success in improving contrast for gray and white matter, which was beneficial for the diagnosis of brain tumour. The demylination in Multiple sclerosis cases was also enhanced in the images. They suggested that ICA could potentially separate out all the tissues which had different relaxation characteristics (different sources of the cocktail party example). This approach thus shows much promise.

In more technical terms : Consider a set of MR frames as a single multispectral image. Where each band is taken during a particular pulse sequence (will be discussed below). Then use ICA on the data to separate out the physiologically independent components. A classifier such as the SVM can improve the contrast further of the separated independent components.

However, using ICA for MRI has been tricky, something I would discuss towards the end of this post and also in future posts.

Before doing so, I intend to touch up on the basics for the sake of completeness.

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Magnetic Resonance Imaging:

I had been thinking of writing a detailed tutorial on MRI, mostly because it requires some basic physics. However I don’t think it is required. I would recommend [4][5][6] for a study of the same in sufficient depth. I have recently taken tutorials on MRI, and would be willing to write for the blog if there are requests.

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An Introduction to Independent Component Analysis:

Independent Component Analysis was developed initially to solve problems such as the cocktail party problem discussed above.

Let’s formalize a problem like the cocktail party example. For simplicity let us assume that there are only two sources and two mixtures (obtained by keeping two recorders at different locations in the party).

Let’s represent these two mixtures as $x_1$ and $x_2$, and let $s_1$ and $s_2$ be the two sources that were mixed. Since we are assuming that the two microphones were kept at different locations, the mixtures $x_1$ and $x_2$ would be different.

We could write this as:

$x_1 = a_{11}s_1 + a_{12}s_2 \quad \cdots \quad (1)$

$x_2 = a_{21}s_1 + a_{22}s_2 \quad \cdots \quad (2)$

The coefficients $a_{11}, a_{12}, a_{21}, a_{22}$ are basically some parameters that depend on the distance of the respective source from the microphones.

Let’s define our problem as : Using only the mixtures $x_i$ estimate the signal sources $s_i$. It is notable that you do not have any knowledge of the parameters $a_{ij}$.

This could be illustrated by this :

Consider three signals:

Suppose we have five mixtures obtained from these three signals.

Signals obtained by mixing source signals

If you only have the mixed signals available. And do not know how they were mixed (parameters $a_{ij}$ not known). And from these mixed signals ($x_{i}$) you have to estimate the source signals ($s_{i}$). This problem is of considerable difficulty.

One approach would be : Use the statistical properties of the signals ($s_i$) to estimate the parameters ($a_{ij}$). It is surprising that it is enough to assume that $s_1$ and $s_2$ are statistically independent. This assumption might not be valid in many scenarios. But works well in most situations.

We could write the above system of linear equations in matrix form as :

$x=As$

where, $A$ represents the mixing matrix, $x$ and $s$ represent the mixtures and the sources respectively.

The problem is to estimate $s$ from $x$ without knowing $A$. The assumption made is that the sources $s$ are statistically independent.

How we go about solving this problem is exciting and an area of active research.  ICA was originally developed for solving such problems. Please follow [12][13][14] for discussions on mutual information, measures of non-gaussianity such as Kurtosis and Negentropy and the fastICA algorithm.

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Why can ICA be used in MRI?

One limitation that ICA faces is that it can not work if more than one signal sources have a  Gaussian distribution. This can be illustrated as follows:

Again consider our equation for just two sources :

$\displaystyle \begin{bmatrix} x_1 \\ x_2 \end{bmatrix} = \begin{bmatrix} a_{11} & a_{12} \\ a_{21} & a_{22} \end{bmatrix} \begin{bmatrix} s_1 \\ s_2 \end{bmatrix}$

Our problem was : We have to estimate $s$ from $x$ without any knowledge of $A$. We would first need to estimate the parameters $A$ from $x$, assuming statistical independence of $s$. And then we could find $s$ as :

$s = Wx$, where $W=A^{-1}$ , or the inverse of the estimated mixing matrix $A$.

To understand how a solution would become impossible if both the sources had a Gaussian distribution, consider this :

Consider two independent components having the following uniform distributions:

$P(s_i) = \begin{cases} \frac{1}{2 \sqrt{3}} & \text{if} \quad |s_i| \leq \sqrt{3} \\ 0 & \text{otherwise} \end{cases}$

The joint density of the two sources would then be uniform on a square. This follows from the fact that the joint density would be the product of the two marginal densities.

The joint distribution for Si

[ Image Source : Reference [12][13] ]

Now if $s_1$ and $s_2$ were mixed by a mixing matrix $A$

$A = \begin{bmatrix} 2 & 3 \\ 2 & 1 \end{bmatrix}$

The mixtures obtained are $x_1$ and $x_2$. Now since the original sources had a joint distribution on a square, and they were transformed by using a mixing matrix, the joint distribution of the mixtures $x_1$ and $x_2$ will be a parallelogram. These mixtures are no longer independent.

Joint Distribution of the mixtures

[ Image Source : Reference [12][13] ]

Now consider the problem once again : We have to estimate the mixing matrix $A$ from the mixtures $x_i$, and using this estimated $A$ we have to estimate the sources $s_i$.

From the above joint distribution we have a way to estimate $A$. The edges of the parallelogram are in a direction given by the columns of $A$. This is an intuitive way of estimating the mixing matrix : obtain the joint distributions of the mixtures, estimate the columns of the mixing matrix by finding the directions of the edges of the parallelogram. This solution gives a good intuitive feel of a in-principle solution of the problem( however, it isn’t practical).

However, now instead of two independent sources having a uniform distribution consider two independent sources having a Gaussian distribution. The joint distribution would be :

Joint Distribution when both Independent sources are Gaussian

[ Image Source : Reference [12][13] ]

Now going by the above discussion, because of the nature of the above joint distribution, it is not possible to estimate the mixing matrix from it.

Thus ICA fails when one or more independent components have a a gaussian distribution.

Noise in MRI is non-gaussian[16], therefore ICA is suited for MRI.

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Problems in Using ICA for MRI Blind Source Separation:

The application of ICA for MRI faces a number of problems. I would discuss these in later blog posts. I would only discuss one major problem – the problem of Over-Complete ICA.

Over-Complete ICA in MRI:

The problem of over complete ICA occurs when there are lesser sensors (tape recorders from our above discussion) than sources. This problem can be understood by the following discussion. Suppose you have 3 mixtures $x_1$, $x_2$ and $x_3$ (imagine you have collected 3 tape recordings in a cocktail party of 6). Therefore you now have to estimate 6 sources from 3 mixtures.

Now the problem becomes something like this :

$x_1 = a_{11}s_1 + a_{12}s_2 + a_{13}s_3 + a_{14}s_4 + a_{15}s_5 + a_{16}s_6$

$x_2 = a_{21}s_1 + a_{22}s_2 + a_{23}s_3 + a_{24}s_4 + a_{25}s_5 + a_{26}s_6$

$x_3 = a_{31}s_1 + a_{32}s_2 + a_{33}s_3 + a_{34}s_4 + a_{35}s_5 + a_{36}s_6$

Assume for a second we can still estimate $a_{ij}$, still we can not find all the signal sources. As the number of linear equations is just three, while the number of unknowns is 6. This is a considerably harder problem and has been discussed by many groups such as [19][20][21].

Now dropping our assumption, the estimation of $a_{ij}$ is also harder in such a case.

The Case in MRI:

The problem of over-complete ICA doesn’t arise when it comes to functional-MRI. However it is a problem when it comes to MRI[17].

In MRI, by varying the parameters used for imaging, the three kind of images that can be obtained are T1 weighted, T2 weighted and Proton Density images. Going by our discussion in the section on MRI above. These three can be treated as mixtures.

Therefore, we have 3 mixtures at our disposal.  However, as the ground truth images above show: The number of different tissues in the brain exceeds 9. Thus this becomes a considerably difficult problem : We have to estimate 9-10 independent components from just 3 mixtures.

I would discuss methods that can help do that in later blog posts.

If only three mixtures are used, 3 ICs can be estimated. Since the actual number of ICs exceeds 9. It is obvious that the each of 3 ICs have atleast 2 ICs mixed, which means that a certain tissue type is not enhanced as much as it could have been had there been one IC for it. This can be understood by looking at this example.

3 ICs obtained by Applying Fast-ICA on MR scans

[I used FastICA for obtaining these Independent Components ]

To get more ICs, in simple words, we need more mixtures. However we can obtain more mixtures from the existing mixtures itself by a process of Band-Expansion[18].

I would discuss this problem of OC-ICA and it’s possible solutions in later posts.

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To Conclude:

A basic idea related to application of ICA to MR scans was discussed. It is clear that even with just three ICs significant tissue contrast enhancement is achieved. Problems related to OC-ICA would be discussed in later posts one by one. I would also discuss quantifying the results obtained using the Tanimoto/Jaccard coefficient of similarity.

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References and Resources:

Cocktail Party Problem

[1] “Some Experiments on the Recognition of Speech, with One and with Two Ears“; E. Colin Cherry; The Journal of the Acoustical Society of America; September 1953. (PDF)

[2] “The Attentive Brain“; Stephen Grossberg; Department of Cognitive and Neural Systemss – Boston University; American Scientist, 1995. (PDF)

[3] “The Cocktail Party Problem : A Primer“; Josh H. McDermott; Current Biology Vol 19. No. 22. (PDF)

Magnetic Resonance Imaging

[4] “Magnetic Resonance ImagingTutorial“; H Panepucci and A Tannus; Technical Report; USP, 1994. (PDF)

[5] “10 Video lessons on MRI by Paul Callaghan” (~ an hour in total). (Videos)

[6] “MRI Tutorial for Neuroscience Boot Camp” Melissa Saenz. (PDF)

Sample ICA Applications Similar to The Cocktail Party Problem

[7] “Independent Component Analysis of Electroencephalographic Data“; Makieng, Bell, Jung, Sejnowski; Advances in Neural Information Processing Systems, 1996. (PDF)

[8] “Application of ICA to MEG noise Reduction“; Masaki Kawakatsu; 4th International Symposium on Independent Component Analysis and Blind Source Separation; 2003. (PDF)

[9] “Independent Component Analysis in Financial Data” from the book Computational Finance; Yasser S. Abu-Mostafa; The MIT Press; 2000. (Book Link)

[10] “ICA of functional MRI data : An overview“; Calhoun, Adali, Hansen, Larsen, Pekar; 4th International Symposium on Independent Component Analysis and Blind Source Separation; 2003. (PDF)

[11] “Independent Component Analysis of fMRI Data – Examining the Assumptions“; McKeown, Sejnowski; Human Brain Mapping; 1998. (PDF)

Independent Component Analysis : Tutorials/Books

[12] “Independent Component Analysis : Algorithms and Applications“; Aapo Hyvärinen, Erkki Oja; Neural Networks; 2000. (PDF)

[13] “Independent Component Analysis“; Aapo Hyvärinen, Juha Karhunen, Erkki Oja; John Wiley Publications; 2001. (Book Link)

[14] ICA Tutorial at videolectures.net by Aapo Hyvärinen. (Videos)

Independent Component Analysis for Magnetic Resonance Imaging

[15] “Application of of Independent Component Analysis to Magnetic Resonance Imaging for enhancing the Contrast of Gray and White Matter“; Nakai, Muraki, Bagarinao, Miki, Takehara, Matsuo, Kato, Sakahara, Isoda; NeuroImage; 2004. (Journal Link)

[16] “Noise in MRI“; Albert Macovski; Magnetic Resonance in Medicine; 1996. (PDF)

[17] “Independent Component Analysis in Magnetic Resonance Image Analysis“;  Ouyang, Chen, Chai, Clayton Chen, Poon, Yang, Lee; EURASIP journal on Advances in Signal Processing; 2008 (Journal Link)

[18] “Band Expansion Based Over-Complete Independent Component Analysis for Multispectral Processing of Magnetic Resonance Images “; Ouyang, Chen, Chai, Clayton Chen, Poon, Yang, Lee; IEEE Transactions on Biomedical Imaging; June 2008. (PDF)

Over-Complete ICA:

[19] “Blind Source Separation of More Sources Than Mixtures Using Over Complete Representations“; Lee, Lewicki, Girolami, Sejnowski; IEEE Signal Processing Letters; 1999. (PDF)

[20] “Learning Overcomplete Representations“; Lewicki, Sejnowski. (PDF)

[21] “A Fast Algorithm for estimating over-complete ICA bases for Image Windows “; Hyvarinen, Cristescu, Oja; International Joint Conference on Neural Networks; 1999. (IEEE Xplore link)

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## Lenfirewood’s Ingenious Digital Artwork

I got linked to by a blog today morning and I wanted to see what it was about. It turned out to be a web-log on the author’s digital photography, compositions of multiple images taken by him and images post processing. Some of the processed images are beautiful. Consider a sample:

The above image christened The Spirit of Autumn is actually a composition of 3 different images taken on a digital camera. The resultant image was then processed further in GIMP (GNU based image manipulation tools) to get a wonderful output.

[Through a Glass Wetly]

I am strongly attracted to painted abstract art, and very rarely to digital art (though I like ingenious fractals and mathematical figures) however this work is truly deserving of a hearty applause. Interestingly, the author pursues photography as a hobby. You can check out some of his other art work at his web-log here.

Image processing is one of my most favorite subjects and I have been involved in projects concerning some Image Processing too, however I think I should move beyond looking at MRI scans or X-rays once in a while and try my hand at post processing photographs taken by me (using tools ofcourse, not algorithms as such) to TRY and get as spectacular results as obtained by DJ Lenfirewood as above. LOL.

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## Kafka to Red Ant: A Strange Metamorphosis

Before I make a start I would want to make it very clear that inspite of what that the title may suggest, this is not a “sensational” post. It is just something that really intrigued me. It basically falls under the domain of image segmentation and pattern recognition, however it is something that can intrigue a person with a non-scientific background with a like (or dislike) for Franz Kafka’s work equally. I keep the title because it is the title of an original work by Dr Vitorino Ramos and hence making changes to it is not a good thing.

Note: For people who are  not interested in technical details can skip those parts and only read the stuff in bold there.

Franz Kafka is one writer whose works have had a profound impact on me in terms that they disturbed me each time I thought about them. No, not because of his writings per se ONLY but for a greater part because i had read a lot on his rather tragic life and i saw a heart breaking reflection in his works of what happened in his life (i see a lot of similarities between Kafka’s life and that of Premchand albeit that Premchand’s work got published in his lifetime mostly, though he got true critical acclaim after his death). Yes i do think that his writings give a good picture of Europe at that time, on human needs and behavior, but the prior reason outweighs all these. Kafka remains one of my favorite writers, though his works are basically short stories. He mostly wrote on a theme that emphasized the alienation of man and the indifferent society. Kafka’s tormenting thoughts on dehumanization, the cruel world, bureaucratic labyrinths which he experienced as being part of the not so liked Jewish minority in Prague, his experiences in jobs he did, his love life and affairs, on a constant fear of mental and physical collapse as a result of clinical depression and the ill health that he suffered from, reflected in a lot of his works. Including in his novella The Metamorphosis.

W. H Auden rightly wrote about Kafka:

“Kafka is important to us because his predicament is the predicament of the modern man”

In metamorphosis the protagonist Gregor Samsa turns into a giant insect when he wakes up one morning. It is kind of apparent that the “transformation” was meant in a metaphorical sense by Kafka and not in a literal one, mostly based on his fears and his own life experiences. The Novella starts like this. . .

As Gregor Samsa awoke one morning from uneasy dreams he found himself transformed in his bed into a monstrous vermin.

While rummaging through a few scientific papers that explored the problem of pattern recognition using a distributed approach i came across a few by Dr Ramos et al, which dealt with the issue using the artificial colonies approach.

In the previous post i had mentioned that the self organization of neurons into a brain like structure and the self organization of an ant colony were similar in more than a few ways. If it may be implemented then it could have implications in pattern recognition problems, where the perceptive abilities emerge from the local and simple interactions of simple agents. Such decentralized systems, a part of the swarm intelligence paradigm look very promising in applying to pattern recognition and the specific case of image segmentation as basically these may be considered a clustering and combinatorial problem taking the image itself as an ant colony habit.

The basis for this post was laid down in the previous post on colony cognitive maps. We observed the evolution of a pheromonal field there and a simple model for the same:

[Evolution of a distribution of (artificial) ants over time: Image Source]

Click to Enlarge

The above is the evolution of the distribution of artificial ants in a square lattice, this work has been extended to digital image lattices by Ramos et al. Image segmentation is an image processing problem wherein the regions of the image under consideration may be partitioned into different regions. Like into areas of low contrast and areas of high contrast, on basis of texture and grey level and so on. Image segmentation is very important as the output of an image segmentation process may be used as an input in object recognition based scenarios. The work of Ramos et al (In references below) and some of the papers cited in his works have really intrigued me and i would strongly suggest readers to have a look at them if at all they are interested in image segmentation, pattern recognition and self organization in general, some might also be interested in implementing something similar too!

In one of the papers a swarm of artificial ants was thrown on a digital habitat (an image of Albert Einstein) to explore it for 1000 iterations. The Einstein image is replaced by a map image. The evolution of the colony cognitive maps for the Einstein image habitat is shown below for various iterations.

[Evolution of a pheromonal field on an Einstein image habitat for t= 0, 1, 100, 110, 120, 130, 150, 200, 300, 400, 500, 800, 900, 1000: Image Source]

The above is represented most aptly in a .gif image.

[Evolution of a pheromonal field on an Einstein habitat: Image Source]

Now instead of Einstein a Kafka image was taken and was subject to the same. Image Source

The Kafka image habitat is replaced by a red ant in the second row. The abstract of the paper by the same name goes as.

Created with an Artificial Ant Colony, that uses images as Habitats, being sensible to their gray levels. At the second row,  Kafka is replaced as a substrate, by Red Ant. In black, the higher levels of pheromone (a chemical evaporative sugar substance used by swarms on their orientation trough out the trails). It’s exactly this artificial evaporation and the computational ant collective group synergy reallocating their upgrades of pheromone at interesting places, that allows for the emergence of adaptation and “perception” of new images. Only some of the 6000 iterations processed are represented. The system does not have any type of hierarchy, and ants communicate only in indirect forms, through out the successive alteration that they found on the Habitat.

Now what intrigues me is that the transition is extremely rapid. Such perceptive ability with change in the image habitat could have massive implications at how we look at pattern recognition for such cases.

Extremely intriguing!

Resources on Franz Kafka:

1. A Brief Biography

3. The Kafka Project

References and STRONGLY recommended papers:

1. Artificial Ant Colonies in Digital Image Habitats – A Mass behavior Effect Study on Pattern Recognition. Vitorino Ramos and Filipe Almeida. Click Here >>

2. Social Cognitive Maps, Swarms Collective Perception and Distributed Search on Dynamic Landscapes. Vitorino Ramos, Carlos Fernandes, Agostinho C. Rosa. Click Here >>

3. Self-Regulated Artificial Ant Colonies on Digital Image Habitats. Carlos Fernandes, Vitorino Ramos, Agostinho C. Rosa. Click Here >>

4. On the Implicit and the Artificial – Morphogenesis and Emergent Aesthetics in Autonomous Collective Systems. Vitorino Ramos. Click Here >>

5. A Strange Metamorphosis [Kafka to Red Ant], Vitorino Ramos.

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